Primary Identifier | MGI:2447783 | Allele Type | Targeted |
Attribute String | Conditional ready, Inserted expressed sequence, Null/knockout | Gene | Ikzf1 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd-Hprt1<b-m3> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A construct containing a floxed neo cassette, Pax5 gene, and GFP gene was inserted into the Znfn1a1 locus upstream of the translation initiation codon in exon 2. Western blot analysis using antibodies against C-terminal Znfn1a1 sequences did not detect protein product in thymocytes from homozygous mutant animals. Trace amounts of Pax5 protein was detected in thymocytes. |