| Primary Identifier | MGI:2447874 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mmp20 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd-Hprt1<b-m3> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Sequence encompassing a portion of intron 4 and all of exon 5 was replaced with a Hprt minigene inserted by homologous recombination. The deleted region encoded the conserved zinc-binding site of the catalytic domain. Northern blot analysis of total RNA obtained from homozygous mutant incisor homogenates indicated a lack of transcript containing exon 5. Enamelysin activity was undetected by zymography of proteins isolated from molars of postnatal homozygous mutant mice. |
