| First Author | Randriamboavonjy V | Year | 2010 |
| Journal | Blood | Volume | 116 |
| Issue | 12 | Pages | 2134-40 |
| PubMed ID | 20558612 | Mgi Jnum | J:164504 |
| Mgi Id | MGI:4834066 | Doi | 10.1182/blood-2010-04-279612 |
| Citation | Randriamboavonjy V, et al. (2010) AMPK alpha2 subunit is involved in platelet signaling, clot retraction, and thrombus stability. Blood 116(12):2134-40 |
| abstractText | The adenosine monophosphate (AMP)-activated protein kinase (AMPK) is a regulator of energy balance at the cellular and whole-body levels, but little is known about the role of AMPK in platelet activation. We report that both the alpha1 and alpha2 AMPK isoforms are expressed by human and murine platelets and that thrombin elicits the phosphorylation of AMPKalpha as well as the upstream kinase, liver kinase B1 (LKB1). In human platelets, the kinase inhibitors iodotubercidin and compound C significantly inhibited thrombin-induced platelet aggregation and clot retraction without affecting the initial increase in [Ca(2+)](i). Clot retraction was also impaired in platelets from AMPKalpha2(-/-) mice but not from wild-type littermates or AMPKalpha1(-/-) mice. Moreover, rebleeding was more frequent in AMPKalpha2(-/-) mice, and the FeCl(3)-induced thrombi formed in AMPKalpha2(-/-) mice were unstable. Mechanistically, AMPKalpha2 was found to phosphorylate in vitro the Src-family kinase, Fyn, and isoform deletion resulted in the attenuated threonine phosphorylation of Fyn as well as the subsequent tyrosine phosphorylation of its substrate, beta3 integrin. These data indicate that AMPKalpha2-by affecting Fyn phosphorylation and activity-plays a key role in platelet alphaIIbbeta3 integrin signaling, leading to clot retraction and thrombus stability. |
