| First Author | Maeoka Y | Year | 2022 |
| Journal | J Am Soc Nephrol | Volume | 33 |
| Issue | 3 | Pages | 584-600 |
| PubMed ID | 35064051 | Mgi Jnum | J:333768 |
| Mgi Id | MGI:7440150 | Doi | 10.1681/ASN.2021081099 |
| Citation | Maeoka Y, et al. (2022) Combined Kelch-like 3 and Cullin 3 Degradation is a Central Mechanism in Familial Hyperkalemic Hypertension in Mice. J Am Soc Nephrol 33(3):584-600 |
| abstractText | BACKGROUND: Mutations in the ubiquitin ligase scaffold protein Cullin 3 (CUL3) gene cause the disease familial hyperkalemic hypertension (FHHt). In the kidney, mutant CUL3 (CUL3-Delta9) increases abundance of With-No-Lysine (K) Kinase 4 (WNK4), inappropriately activating sterile 20/SPS-1-related proline/alanine-rich kinase (SPAK), which then phosphorylates and hyperactivates the Na(+)Cl(-) cotransporter (NCC). The precise mechanism by which CUL3-Delta9 causes FHHt is unclear. We tested the hypothesis that reduced abundance of CUL3 and of Kelch-like 3 (KLHL3), the CUL3 substrate adaptor for WNK4, is mechanistically important. Because JAB1, an enzyme that inhibits CUL3 activity by removing the ubiquitin-like protein NEDD8, cannot interact with CUL3-Delta9, we also determined whether Jab1 disruption mimicked the effects of CUL3-Delta9 expression. METHODS: We used an inducible renal tubule-specific system to generate several mouse models expressing CUL3-Delta9, mice heterozygous for both CUL3 and KLHL3 (Cul3(+/-)/Klhl3(+/-) ), and mice with short-term Jab1 disruption (to avoid renal injury associated with long-term disruption). RESULTS: Renal KLHL3 was higher in Cul3(-/-) mice, but lower in Cul3(-/-/Delta9) mice and in the Cul3(+/-/Delta9) FHHt model, suggesting KLHL3 is a target for both WT and mutant CUL3. Cul3(+/-)/Klhl3(+/-) mice displayed increased WNK4-SPAK activation and phospho-NCC abundance and an FHHt-like phenotype with increased plasma [K(+)] and salt-sensitive blood pressure. Short-term Jab1 disruption in mice lowered the abundance of CUL3 and KLHL3 and increased the abundance of WNK4 and phospho-NCC. CONCLUSIONS: Jab1(-/-) mice and Cul3(+/-)/Klhl3(+/-) mice recapitulated the effects of CUL3-Delta9 expression on WNK4-SPAK-NCC. Our data suggest degradation of both KLHL3 and CUL3 plays a central mechanistic role in CUL3-Delta9-mediated FHHt. |
