| First Author | Kim H | Year | 2018 |
| Journal | Sci Rep | Volume | 8 |
| Issue | 1 | Pages | 17642 |
| PubMed ID | 30518775 | Mgi Jnum | J:268037 |
| Mgi Id | MGI:6270267 | Doi | 10.1038/s41598-018-36097-6 |
| Citation | Kim H, et al. (2018) Generation of a highly efficient and tissue-specific tryptophan hydroxylase 1 knockout mouse model. Sci Rep 8(1):17642 |
| abstractText | Recent studies on tissue-autonomous serotonin (5-hydroxytryptamine [5-HT]) function have identified new roles for 5-HT in peripheral organs. Most of these studies were performed by crossing mice carrying the Tph1(tm1Kry) allele with tissue specific Cre mice. In the present study, we found that 5-HT production was not completely abolished in Tph1(tm1Kry) KO mice. The residual 5-HT production in Tph1(tm1Kry) KO mice is attributed to the expression of a truncated form of TPH1 containing the catalytic domain. Hence, in an effort to obtain mice with a Tph1 null phenotype, we generated mice harboring a new Tph1 floxed allele, Tph1(tm1c), targeting exons 5 and 6 which encode the catalytic domain of TPH1. By crossing the new Tph1 floxed mice with villin-Cre or insulin-Cre mice, we observed near-complete ablation of 5-HT production in the intestine and beta cells. In conclusion, this improved Tph1 floxed mouse model will serve as useful and accurate tool for analyzing peripheral 5-HT system. |
