| Primary Identifier | MGI:2448972 | Allele Type | Targeted |
| Attribute String | No functional change, Recombinase | Gene | Nkx2-5 |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | Sequence encoding cre recombinase adjoined to an IRES was inserted into the 3' UTR of the endogenous locus via homologous recombination. A single frt site remained in the UTR after an frt-flanked hygromycin selection cassette was excised. A lack of observed allele complementation indicated that the inserted sequence did not significantly affect expression of the endogenous locus. |
