| First Author | Zigrino P | Year | 2012 |
| Journal | Eur J Cell Biol | Volume | 91 |
| Issue | 10 | Pages | 748-56 |
| PubMed ID | 22717126 | Mgi Jnum | J:240452 |
| Mgi Id | MGI:5883469 | Doi | 10.1016/j.ejcb.2012.05.003 |
| Citation | Zigrino P, et al. (2012) Loss of epidermal MMP-14 expression interferes with angiogenesis but not with re-epithelialization. Eur J Cell Biol 91(10):748-56 |
| abstractText | Synthesis and activation of matrix metalloproteinases during wound healing are important for remodeling the extracellular matrix and modulating various cellular functions. The membrane-type 1 matrix metalloproteinase (MMP-14) has been shown to play a key role during these processes. To analyze the function of epidermal-derived MMP-14 during skin repair we generated mice lacking MMP-14 expression in the epidermis (MMP-14(ep-/-)). These mice displayed overall normal skin morphology and epidermal differentiation patterns. Wound repair in MMP-14(ep-/-) followed the same kinetics as in wild type mice (MMP-14(ep+/+)), and infiltration of neutrophils, leukocytes, and macrophages into the wound site was comparable. Microscopic analysis showed no altered re-epithelialization in the absence of epidermal MMP-14. Furthermore, epidermal differentiation at the end of the repair process and scar formation was normal. However, at day 14 post wounding, sustained angiogenesis was observed in MMP-14(ep-/-) mice in contrast to control mice. Interestingly, decreased levels of endostatin were detected in wound lysates of MMP-14(ep-/-) mice as well as in cultured keratinocytes. Taken together, these data indicate that MMP-14 expression in keratinocytes is dispensable for skin homeostasis and repair, but plays a crucial role in the epidermal-dermal crosstalk leading to modulation of vessel density. |
