| First Author | Tan K | Year | 2020 |
| Journal | Elife | Volume | 9 |
| PubMed ID | 32773035 | Mgi Jnum | J:298586 |
| Mgi Id | MGI:6477041 | Doi | 10.7554/eLife.57525 |
| Citation | Tan K, et al. (2020) The role of the NMD factor UPF3B in olfactory sensory neurons. Elife 9:e57525 |
| abstractText | The UPF3B-dependent branch of the nonsense-mediated RNA decay (NMD) pathway is critical for human cognition. Here, we examined the role of UPF3B in the olfactory system. Single-cell RNA-sequencing (scRNA-seq) analysis demonstrated considerable heterogeneity of olfactory sensory neuron (OSN) cell populations in wild-type (WT) mice, and revealed that UPF3B loss influences specific subsets of these cell populations. UPF3B also regulates the expression of a large cadre of antimicrobial genes in OSNs, and promotes the selection of specific olfactory receptor (Olfr) genes for expression in mature OSNs (mOSNs). RNA-seq and Ribotag analyses identified classes of mRNAs expressed and translated at different levels in WT and Upf3b-null mOSNs. Integrating multiple computational approaches, UPF3B-dependent NMD target transcripts that are candidates to mediate the functions of NMD in mOSNs were identified in vivo. Together, our data provides a valuable resource for the olfactory field and insights into the roles of NMD in vivo. |
