| First Author | Yang JL | Year | 2023 |
| Journal | Neural Regen Res | Volume | 18 |
| Issue | 7 | Pages | 1521-1526 |
| PubMed ID | 36571357 | Mgi Jnum | J:354601 |
| Mgi Id | MGI:7658019 | Doi | 10.4103/1673-5374.357910 |
| Citation | Yang JL, et al. (2023) Transient neurogenesis in ischemic cortex from Sox2(+) astrocytes. Neural Regen Res 18(7):1521-1526 |
| abstractText | The adult cortex has long been regarded as non-neurogenic. Whether injury can induce neurogenesis in the adult cortex is still controversial. Here, we report that focal ischemia stimulates a transient wave of local neurogenesis. Using 5'-bromo-2'-deoxyuridine labeling, we demonstrated a rapid generation of doublecortin-positive neuroblasts that died quickly in mouse cerebral cortex following ischemia. Nestin-Cre(ER)-based cell ablation and fate mapping showed a small contribution of neuroblasts by subventricular zone neural stem cells. Using a mini-photothrombotic ischemia mouse model and retrovirus expressing green fluorescent protein labeling, we observed maturation of locally generated new neurons. Furthermore, fate tracing analyses using PDGFRalpha-, GFAP-, and Sox2-Cre(ER) mice showed a transient wave of neuroblast generation in mild ischemic cortex and identified that Sox2-positive astrocytes were the major neurogenic cells in adult cortex. In addition, a similar upregulation of Sox2 and appearance of neuroblasts were observed in the focal ischemic cortex of Macaca mulatta. Our findings demonstrated a transient neurogenic response of Sox2-positive astrocytes in ischemic cortex, which suggests the possibility of inducing neuronal regeneration by amplifying this intrinsic response in the future. |
