| First Author | Joppé SE | Year | 2020 |
| Journal | Life Sci Alliance | Volume | 3 |
| Issue | 7 | PubMed ID | 32482782 |
| Mgi Jnum | J:337341 | Mgi Id | MGI:6729564 |
| Doi | 10.26508/lsa.202000743 | Citation | Joppe SE, et al. (2020) Genetic targeting of neurogenic precursors in the adult forebrain ventricular epithelium. Life Sci Alliance 3(7) |
| abstractText | The ventricular epithelium of the adult forebrain is a heterogeneous cell population that is a source of both quiescent and activated neural stem cells (qNSCs and aNSCs, respectively). We genetically targeted a subset of ventricle-contacting, glial fibrillary acidic protein (GFAP)-expressing cells, to study their involvement in qNSC/aNSC-mediated adult neurogenesis. Ventricle-contacting GFAP(+) cells were lineage-traced beginning in early adulthood using adult brain electroporation and produced small numbers of olfactory bulb neuroblasts until at least 21 mo of age. Notably, electroporated GFAP(+) neurogenic precursors were distinct from both qNSCs and aNSCs: they did not give rise to neurosphere-forming aNSCs in vivo or after extended passaging in vitro and they were not recruited during niche regeneration. GFAP(+) cells with these properties included a FoxJ1(+)GFAP(+) subset, as they were also present in an inducible FoxJ1 transgenic lineage-tracing model. Transiently overexpressing Mash1 increased the neurogenic output of electroporated GFAP(+) cells in vivo, identifying them as a potentially recruitable population. We propose that the qNSC/aNSC lineage of the adult forebrain coexists with a distinct, minimally expanding subset of GFAP(+) neurogenic precursors. |
