| First Author | Henderson F | Year | 2024 |
| Journal | Cell Rep | Volume | 43 |
| Issue | 7 | Pages | 114411 |
| PubMed ID | 38944834 | Mgi Jnum | J:353077 |
| Mgi Id | MGI:7709015 | Doi | 10.1016/j.celrep.2024.114411 |
| Citation | Henderson F, et al. (2024) Regulation of stress-induced sleep perturbations by dorsal raphe VGLUT3 neurons in male mice. Cell Rep 43(7):114411 |
| abstractText | Exposure to stressors has profound effects on sleep that have been linked to serotonin (5-HT) neurons of the dorsal raphe nucleus (DR). However, the DR also comprises glutamatergic neurons expressing vesicular glutamate transporter type 3 (DR(VGLUT3)), leading us to examine their role. Cell-type-specific tracing revealed that DR(VGLUT3) neurons project to brain areas regulating arousal and stress. We found that chemogenetic activation of DR(VGLUT3) neurons mimics stress-induced sleep perturbations. Furthermore, deleting VGLUT3 in the DR attenuated stress-induced sleep perturbations, especially after social defeat stress. In the DR, VGLUT3 is found in subsets of 5-HT and non-5-HT neurons. We observed that both populations are activated by acute stress, including those projecting to the ventral tegmental area. However, deleting VGLUT3 in 5-HT neurons minimally affected sleep regulation. These findings suggest that VGLUT3 expression in the DR drives stress-induced sleep perturbations, possibly involving non-5-HT DR(VGLUT3) neurons. |
