| Primary Identifier | MGI:2449060 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(TAG-Tcra,TAG-Tcrb)1Dh |
| Strain of Origin | (C57BL/6 x DBA/2)F2 | Is Recombinase | false |
| Is Wild Type | false |
| description | This allele is refered to as Tag-TCR1 by the authors. Tag-TCR1 animals carry 2 copies of Tcrb and 1 copy of Tcra integrated into the same locus. |
| molecularNote | Two transgene constructs were used in the generation of this allele. One construct consists of a Tcra sequence encoding the Valpha2JalphaMT1-27 rearrangement, 6 kb of 5' flanking sequence, 3.8 kb of 3' flanking sequence, and a neomycin resistance gene. The other construct consists of a Tcrb sequence encoding the Vbeta8.3DJbeta2.4 rearrangement, 5.1 kb of 5' flanking sequence, 0.4 kb of 3' flanking sequence, and a neomycin resistance gene. This transgenic line carries two copies of the Tcrb construct and one copy of the Tcra construct cointegrated at one locus. |
