| First Author | Zhang S | Year | 2022 |
| Journal | Science | Volume | 378 |
| Issue | 6623 | Pages | eabo5503 |
| PubMed ID | 36454848 | Mgi Jnum | J:331892 |
| Mgi Id | MGI:7407369 | Doi | 10.1126/science.abo5503 |
| Citation | Zhang S, et al. (2022) Monitoring of cell-cell communication and contact history in mammals. Science 378(6623):eabo5503 |
| abstractText | Monitoring of cell-cell communication in multicellular organisms is fundamental to understanding diverse biological processes such as embryogenesis and tumorigenesis. To track cell-cell contacts in vivo, we developed an intercellular genetic technology to monitor cell-cell contact and to trace cell contact histories by permanently marking contacts between cells. In mice, we engineered an artificial Notch ligand into one cell (the sender cell) and an artificial receptor into another cell (the receiver cell). Contact between the sender and receiver cells triggered a synthetic Notch signaling that activated downstream transcriptional programs in the receiver cell, thereby transiently or permanently labeling it. In vivo cell-cell contact was observed during development, tissue homeostasis, and tumor growth. This technology may be useful for studying dynamic in vivo cell-cell contacts and cell fate plasticity. |
