| First Author | Peng Y | Year | 2023 |
| Journal | Int J Mol Sci | Volume | 24 |
| Issue | 3 | PubMed ID | 36768713 |
| Mgi Jnum | J:355304 | Mgi Id | MGI:7436429 |
| Doi | 10.3390/ijms24032391 | Citation | Peng Y, et al. (2023) Egln1(Tie2Cre) Mice Exhibit Similar Therapeutic Responses to Sildenafil, Ambrisentan, and Treprostinil as Pulmonary Arterial Hypertension (PAH) Patients, Supporting Egln1(Tie2Cre) Mice as a Useful PAH Model. Int J Mol Sci 24(3) |
| abstractText | Pulmonary arterial hypertension (PAH) is a progressive and inevitably fatal disease characterized by the progressive increase of pulmonary vascular resistance and obliterative pulmonary vascular remodeling, which lead to right-sided heart failure and premature death. Many of the genetically modified mouse models do not develop severe PH and occlusive vascular remodeling. Egln1(Tie2Cre) mice with Tie2Cre-mediated deletion of Egln1, which encodes hypoxia-inducible factor (HIF) prolyl hydroxylase 2 (PHD2), is the only mouse model with severe PAH, progressive occlusive pulmonary vascular remodeling, and right-sided heart failure leading to 50-80% mortality from the age of 3-6 months, indicating that the Egln1(Tie2Cre) mice model is a long-sought-after murine PAH model. However, it is unknown if Egln1(Tie2Cre) mice respond to FDA-approved PAH drugs in a way similar to PAH patients. Here, we tested the therapeutic effects of the three vasodilators: sildenafil (targeting nitric oxide signaling), ambrisentan (endothelin receptor antagonist), and treprostinil (prostacyclin analog) on Egln1(Tie2Cre) mice. All of them attenuated right ventricular systolic pressure (RVSP) in Egln1(Tie2Cre) mice consistent with their role as vasodilators. However, these drugs have no beneficial effects on pulmonary arterial function. Cardiac output was also markedly improved in Egln1(Tie2Cre) mice by any of the drug treatments. They only partially improved RV function and reduced RV hypertrophy and pulmonary vascular remodeling as well as improving short-term survival in a drug-dependent manner. These data demonstrate that Egln1(Tie2Cre) mice exhibit similar responses to these drugs as PAH patients seen in clinical trials. Thus, our study provides further evidence that the Egln1(Tie2Cre) mouse model of severe PAH is an ideal model of PAH and is potentially useful for enabling identification of drug targets and preclinical testing of novel PAH drug candidates. |
