| First Author | Erlandsson L | Year | 2001 |
| Journal | J Exp Med | Volume | 194 |
| Issue | 5 | Pages | 557-70 |
| PubMed ID | 11535625 | Mgi Jnum | J:71355 |
| Mgi Id | MGI:2149868 | Doi | 10.1084/jem.194.5.557 |
| Citation | Erlandsson L, et al. (2001) Joining chain-expressing and -nonexpressing B cell populations in the mouse. J Exp Med 194(5):557-70 |
| abstractText | The diphtheria toxin A chain (DTA) was gene targeted into the Joining chain (J chain) locus to create a mouse strain selecting against J chain-expressing cells, JDTA mice. Serum immunoglobulin (Ig)M and serum IgG were reduced six to eightfold, while serum IgA was elevated 14-fold in these mice. JDTA mice were immune competent although the serum Ig response compared with wild-type mice was reduced sixfold at day 14 but only fourfold at day 45 after immunization. Exchanging the DTA gene with a cDNA for c-myc resulted in mice with a distinct phenotype with increased Ig production and enhanced humoral immune responses. Analysis of single B cells stimulated by lipopolysaccharide in vitro using reverse transcription-polymerase chain reaction showed that J chain-nonexpressing B cells could be detected that had a secretory phenotype as determined by an abundance of transcript for secretory IgM. Finally, limiting dilution analysis of peripheral B cells showed that J chain expression was a clonal property already established in naive, peripheral B lymphocytes. |
