| First Author | Arnold P | Year | 2017 |
| Journal | Cell Tissue Res | Volume | 367 |
| Issue | 2 | Pages | 351-358 |
| PubMed ID | 27628095 | Mgi Jnum | J:326936 |
| Mgi Id | MGI:6757789 | Doi | 10.1007/s00441-016-2498-3 |
| Citation | Arnold P, et al. (2017) Deficiency of the DSPP-cleaving enzymes meprin alpha and meprin beta does not result in dentin malformation in mice. Cell Tissue Res 367(2):351-358 |
| abstractText | Formation of dentin requires the maturation of procollagen I and the proteolytic processing of the dentin sialophosphoprotein (DSPP). These cleavage events can be facilitated by the metalloproteinases meprin alpha and meprin beta as well as by bone morphogenetic protein 1 (BMP-1). All three enzymes have been shown to play important roles during collagen I maturation in vivo and their potential in cleaving DSPP was demonstrated in vitro. Hence, it has been discussed whether meprin alpha, meprin beta, BMP-1 or all three are crucial factors in the onset and progression of dentin-related diseases and this issue is addressed here. In this study, we compare the incisors and molars of meprin alpha (Mep1a (-/-))- and meprin beta (Mep1b (-/-))-deficient mice with wild-type (WT) controls on the macroscopic and microscopic level. The dentin was evaluated towards the bone mineral density, dentin volume, calcification and collagen matrix integrity. Using immunohistochemistry, we could identify meprin beta, BMP-1 and DSPP/DSP in the pre-dentin of WT mice. Nevertheless, no significant dentin malformation was observed in Mep1b (-/-) or Mep1a (-/-) deficient mice. |
