| First Author | Dzudzor B | Year | 2010 |
| Journal | Mol Cell Neurosci | Volume | 43 |
| Issue | 1 | Pages | 108-16 |
| PubMed ID | 19837165 | Mgi Jnum | J:158323 |
| Mgi Id | MGI:4438545 | Doi | 10.1016/j.mcn.2009.09.012 |
| Citation | Dzudzor B, et al. (2010) Regulated expression of the Ras effector Rin1 in forebrain neurons. Mol Cell Neurosci 43(1):108-16 |
| abstractText | The Ras effector Rin1 is induced concomitant with synaptogenesis in forebrain neurons, where it inhibits fear conditioning and amygdala LTP. In epithelial cells, lower levels of Rin1 orchestrate receptor endocytosis. A 945 bp Rin1 promoter fragment was active in hippocampal neurons and directed accurate tissue-specific and temporal expression in transgenic mice. Regulated expression in neurons and epithelial cells was mediated in part by Snail transcriptional repressors: mutation of a conserved Snail site increased expression and endogenous Snai1 was detected at the Rin1 promoter. We also describe an element closely related to, but distinct from, the consensus site for REST, a master repressor of neuronal genes. Conversion to a consensus REST sequence reduced expression in both cell types. These results provide insight into regulated expression of a neuronal Ras effector, define a promoter useful in telencephalic neuron studies, and describe a novel REST site variant directing expression to mature neurons. |
