| Primary Identifier | MGI:2651420 | Allele Type | Targeted |
| Gene | Oprm1 | Transmission | Germline |
| Strain of Origin | Not Specified | Is Recombinase | false |
| Is Wild Type | false |
| description | ES cell line = SP1. |
| molecularNote | The intronic neo cassette was excised from Oprmtm2Loh via in vivo cre mediated recombination, leaving the engineered serine to alanine missense mutation in exon 2 at codon 196 (S196A) intact. S196A affects the fourth transmembrane domain. A radioactive ligand receptor-binding assay showed reduced expression of the mutant protein in homozygous mutant mice, approximately 85% that of the endogenous protein in wild-type mice. The reduced protein expression correlated with reduced levels of transcript. |
