| First Author | McCartney SA | Year | 2008 |
| Journal | PLoS Pathog | Volume | 4 |
| Issue | 7 | Pages | e1000108 |
| PubMed ID | 18636103 | Mgi Jnum | J:162720 |
| Mgi Id | MGI:4819674 | Doi | 10.1371/journal.ppat.1000108 |
| Citation | McCartney SA, et al. (2008) MDA-5 recognition of a murine norovirus. PLoS Pathog 4(7):e1000108 |
| abstractText | Noroviruses are important human pathogens responsible for most cases of viral epidemic gastroenteritis worldwide. Murine norovirus-1 (MNV-1) is one of several murine noroviruses isolated from research mouse facilities and has been used as a model of human norovirus infection. MNV-1 infection has been shown to require components of innate and adaptive immunity for clearance; however, the initial host protein that recognizes MNV-1 infection is unknown. Because noroviruses are RNA viruses, we investigated whether MDA5 and TLR3, cellular sensors that recognize dsRNA, are important for the host response to MNV-1. We demonstrate that MDA5-/- dendritic cells(DC) have a defect in cytokine response to MNV-1. In addition, MNV-1 replicates to higher levels in MDA5-/- DCs as well as in MDA5-/- mice in vivo. Interestingly, TLR3-/- DCs do not have a defect in vitro, but TLR3-/- mice have a slight increase in viral titers. This is the first demonstration of an innate immune sensor for norovirus and shows that MDA5 is required for the control of MNV-1 infection. Knowledge of the host response to MNV-1 may provide keys for prevention and treatment of the human disease. |
