| Primary Identifier | MGI:2653294 | Allele Type | Targeted |
| Gene | Lepr | Transmission | Germline |
| Strain of Origin | 129/Sv | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Tyrosine 1138 of exon 18b was replaced with a serine residue (Y1138S) and a neomycin resistance cassette was inserted into the downstream intron via homologous recombination. The Y1138S amino acid substitution specifically disrupts the leptin receptor long form (LRb)-STAT3 signal. Expression of the knock-in allele in mutant animals was confirmed by semi-quantitative RT-PCR analysis of hypothalamus RNA. |
