| Primary Identifier | MGI:2653307 | Allele Type | Targeted |
| Attribute String | Modified regulatory region, Null/knockout | Gene | Rr133 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 754 bp region, containing the 208 bp branchial arch enhancer upstream of the Hand2 gene, was replaced with a loxP site flanked neomycin resistance gene cassette, which was removed through subsequent cre-mediated recombination. Whole mount in situ hybridization of homozygous mutant embryos showed a lack of endogenous transcript in the ventrolateral portions of the first and second branchial arches. |
