| First Author | Kanamoto T | Year | 2011 |
| Journal | Curr Eye Res | Volume | 36 |
| Issue | 4 | Pages | 336-40 |
| PubMed ID | 21405954 | Mgi Jnum | J:179792 |
| Mgi Id | MGI:5303053 | Doi | 10.3109/02713683.2011.556301 |
| Citation | Kanamoto T, et al. (2011) Platelet-derived growth factor receptor alpha is associated with oxidative stress-induced retinal cell death. Curr Eye Res 36(4):336-40 |
| abstractText | PURPOSE: The purpose of this study was to investigate the role played by platelet-derived growth factor-alpha (PDGFRalpha) in oxidative stress-induced retinal cell death. A previous proteomic study from our laboratory showed that expression of PDGFRalpha is elevated considerably in the retinas of an animal model of glaucoma-the excitatory amino acid carrier (EAAC) 1-deficient (EAAC1-/-) mouse. METHODS: Retinal sites and expression patterns of PDGFRalpha were determined by immunohistochemistry in the retinas of EAAC1-/- and control CRL:CD1(ICR) mice. A retinal cell line was exposed to hydrogen peroxide, and expression PDGFRalpha determined by Western blot analysis. Effects of PDGF-AA and PDGFRalpha-siRNA on hydrogen peroxide-induced retinal cell death were examined. RESULTS: PDGFRalpha was detected in the retinal ganglion cell layer (RGL) of both EAAC1-/- and ICR mice, and was also localized in the internal nuclear layer (INL) of EAAC1-/- mice. While treatment with excess PDGF-AA had no additional effect on retinal cell death, expression of PDGFRalpha increased with exposure to hydrogen peroxide. Hydrogen peroxide-induced retinal cell death was inhibited by exposure to PDGF-AA via phosphatidylinositol 3 kinase (PI3K); cell death was promoted by PDGFRalpha-siRNA. CONCLUSIONS: PDGFRalpha is expressed in mouse retina, where it is essential for retinal cell survival under conditions of oxidative stress. |
