| Primary Identifier | MGI:2654351 | Allele Type | Targeted |
| Attribute String | Modified isoform(s), Null/knockout | Gene | Wt1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A nonsense mutation was introduced into exon 1, 12 codons downstream of the CUG start codon and upstream of the AUG start codon. A floxed hygro cassette was excised in vitro, leaving a single loxP site in a nonconserved region within in intron 1. Western blot analysis of homozygous mutant fetal kidney and testis tissue showed that while the CUG-initiated isoforms were undetected, normal levels of AUG-initiated isoforms were present. |
