| First Author | Ikeda M | Year | 2000 |
| Journal | Neuroreport | Volume | 11 |
| Issue | 5 | Pages | 907-12 |
| PubMed ID | 10790853 | Mgi Jnum | J:103572 |
| Mgi Id | MGI:3610426 | Doi | 10.1097/00001756-200004070-00002 |
| Citation | Ikeda M, et al. (2000) PLC beta 4-independent Ca2+ rise via muscarinic receptors in the mouse suprachiasmatic nucleus. Neuroreport 11(5):907-12 |
| abstractText | Cholinergic regulation of the suprachiasmatic nucleus (SCN) has been extensively studied although the intracellular signaling mechanisms are not well understood. We examined immunostaining for phospholipase C-beta (PLC-beta) families that couple to muscarinic acetylcholine receptors (mAChR) and demonstrated the expression of PLC-beta 1 and beta 4 in the mouse SCN. Ca2+ imaging analysis indicated that the MI-mAChR antagonist, pirenzepine blocked carbachol-induced Ca2+ elevation in the SCN and the response was equivalent between the wild type and the PLC-beta 4-knockout mice. In addition, the knockout mice displayed locomotor and temperature rhythms coupling to 24 h light/dark cycles. Therefore, it was proposed that PLC-beta 1 but not PLC-beta 4 was involved in the mAChR-mediated Ca2+ signaling in the SCN. |
