| First Author | Duesman SJ | Year | 2023 |
| Journal | Front Immunol | Volume | 14 |
| Pages | 1088039 | PubMed ID | 36855628 |
| Mgi Jnum | J:334119 | Mgi Id | MGI:7440693 |
| Doi | 10.3389/fimmu.2023.1088039 | Citation | Duesman SJ, et al. (2023) Transforming growth factor receptor III (Betaglycan) regulates the generation of pathogenic Th17 cells in EAE. Front Immunol 14:1088039 |
| abstractText | The transforming growth factor receptor III (TbetaRIII) is commonly recognized as a co-receptor that promotes the binding of TGFbeta family ligands to type I and type II receptors. Within the immune system, TbetaRIII regulates T cell development in the thymus and is differentially expressed through activation; however, its function in mature T cells is unclear. To begin addressing this question, we developed a conditional knock-out mouse with restricted TbetaRIII deletion in mature T cells, necessary because genomic deletion of TbetaRIII results in perinatal mortality. We determined that TbetaRIII null mice developed more severe autoimmune central nervous neuroinflammatory disease after immunization with myelin oligodendrocyte peptide (MOG(35-55)) than wild-type littermates. The increase in disease severity in TbetaRIII null mice was associated with expanded numbers of CNS infiltrating IFNgamma(+) CD4(+) T cells and cells that co-express both IFNgamma and IL-17 (IFNgamma(+)/IL-17(+)), but not IL-17 alone expressing CD4 T cells compared to Tgfbr3(fl/fl) wild-type controls. This led us to speculate that TbetaRIII may be involved in regulating conversion of encephalitogenic Th17 to Th1. To directly address this, we generated encephalitogenic Th17 and Th1 cells from wild type and TbetaRIII null mice for passive transfer of EAE into naive mice. Remarkably, Th17 encephalitogenic T cells from TbetaRIII null induced EAE of much greater severity and earlier in onset than those from wild-type mice. The severity of EAE induced by encephalitogenic wild-type and Tgfbr3(fl/fl).dLcKCre Th1 cells were similar. Moreover, in vitro restimulation of in vivo primed Tgfbr3(fl/fl).dLcKCre T cells, under Th17 but not Th1 polarizing conditions, resulted in a significant increase of IFNgamma(+) T cells. Altogether, our data indicate that TbetaRIII is a coreceptor that functions as a key checkpoint in controlling the pathogenicity of autoreactive T cells in neuroinflammation probably through regulating plasticity of Th17 T cells into pathogenic Th1 cells. Importantly, this is the first demonstration that TbetaRIII has an intrinsic role in T cells. |
