| Primary Identifier | MGI:2657151 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Dnmt1 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| description | Previously targeted J1 ES cells homozygous for Dnmt1tm1Enl were electroporated with the targeting vector. |
| molecularNote | A targeting vector containing a Dnmt1 cDNA fragment followed by a PGK-puro cassette was inserted upstream of the previously targeted coding region containing a neomycin selection cassette (Dnmt1tm1Enl) and downstream of the endogenous promoter. Northern blot analysis showed that transcript produced by the inserted cDNA sequence was at a level and size similar to that of the endogenous allele. |
