| First Author | Takeuchi J | Year | 2002 |
| Journal | Pharmacol Biochem Behav | Volume | 72 |
| Issue | 1-2 | Pages | 117-23 |
| PubMed ID | 11900778 | Mgi Jnum | J:83217 |
| Mgi Id | MGI:2658775 | Doi | 10.1016/s0091-3057(01)00725-0 |
| Citation | Takeuchi J, et al. (2002) Increased drinking in mutant mice with truncated M5 muscarinic receptor genes. Pharmacol Biochem Behav 72(1-2):117-23 |
| abstractText | The rarest and least understood of the muscarinic receptors is the M5 subtype. Recombinant methods were used to create mutant mice with a deletion in the third intracellular loop of the M5 receptor gene. Salivation induced by the nonselective muscarinic agonist pilocarpine (1 mg/kg sc) was reduced in homozygous mutants from 15 to 60 min after injection as compared with wild-type mice. After 18-h food and water deprivation, drinking was increased in homozygous mutants, but feeding was not increased. The mutant and wild-type mice had similar responses in tests of open-field exploration, seizures induced by pilocarpine (300 mg/kg) or hypothermia induced by pilocarpine (1-3 mg/kg). These results indicate that M5 muscarinic receptors are important for fluid intake and suggest that M5 receptors are involved in slow secretory processes. |
