Other
14 Authors
- Park C,
- Wei L,
- Lee MY,
- Sanders KM,
- Zogg H,
- Kim S,
- Poudrier SM,
- Singh R,
- Jin B,
- Jorgensen BG,
- Ha SE,
- Kurahashi M,
- Ro S,
- Baek G
| First Author | Ha SE | Year | 2022 |
| Journal | PLoS One | Volume | 17 |
| Issue | 5 | Pages | e0261743 |
| PubMed ID | 35560163 | Mgi Jnum | J:327724 |
| Mgi Id | MGI:7280295 | Doi | 10.1371/journal.pone.0261743 |
| Citation | Ha SE, et al. (2022) Transcriptome profiling of subepithelial PDGFRalpha cells in colonic mucosa reveals several cell-selective markers. PLoS One 17(5):e0261743 |
| abstractText | Subepithelial platelet-derived growth factor receptor alpha (PDGFRalpha)+ cells found in the colonic mucosal tissue come in close contact with epithelial cells, immune cells, neurons, capillaries, and lymphatic networks. Mucosal subepithelial PDGFRalpha+ cells (MuPalphaC) are important regulators in various intestinal diseases including fibrosis and inflammation. However, the transcriptome of MuPalphaC has not yet been elucidated. Using Pdgfra-eGFP mice and flow cytometry, we isolated colonic MuPalphaC and obtained their transcriptome data. In analyzing the transcriptome, we identified three novel, and selectively expressed, markers (Adamdec1, Fin1, and Col6a4) found in MuPalphaC. In addition, we identified a unique set of MuPalphaC-enriched genetic signatures including groups of growth factors, transcription factors, gap junction proteins, extracellular proteins, receptors, cytokines, protein kinases, phosphatases, and peptidases. These selective groups of genetic signatures are linked to the unique cellular identity and function of MuPalphaC. Furthermore, we have added this MuPalphaC transcriptome data to our Smooth Muscle Genome Browser that contains the transcriptome data of jejunal and colonic smooth muscle cells (SMC), interstitial cells of Cajal (ICC), and smooth muscle resident PDGFRalpha+ cells: (https://med.unr.edu/physio/transcriptome). This online resource provides a comprehensive reference of all currently known genetic transcripts expressed in primary MuPalphaC in the colon along with smooth muscle resident PDGFRalpha cells, SMC, and ICC in the murine colon and jejunum. |
