| First Author | Okada A | Year | 2019 |
| Journal | Biochem Biophys Res Commun | Volume | 512 |
| Issue | 2 | Pages | 326-330 |
| PubMed ID | 30890337 | Mgi Jnum | J:291182 |
| Mgi Id | MGI:6442800 | Doi | 10.1016/j.bbrc.2019.03.033 |
| Citation | Okada A, et al. (2019) Transcriptionally distinct mesenchymal stem/stromal cells circulate in fetus. Biochem Biophys Res Commun 512(2):326-330 |
| abstractText | Umbilical cord blood contains mesenchymal stem/stromal cells (MSCs) in addition to hematopoietic stem cells, serving as an attractive tool for regenerative medicine. As umbilical cord blood originates from fetus, abundant MSCs are expected to circulate in fetus. However, the properties of circulating MSCs in fetus have not been fully examined. In the present study, we aimed to analyze circulating MSCs, marked by the expression of platelet-derived growth factor receptor alpha (PDGFRalpha), during fetal development. Using PDGFRalpha GFP knock-in mice, we quantified the number of circulating PDGFRalpha positive MSCs during development. We further performed whole transcriptome analysis of circulating MSCs at single cell levels. We found that abundant PDGFRalpha positive cells circulate in embryo and diminish immediately after birth. In addition, single cell RNA-sequencing revealed transcriptional heterogeneity of MSCs in fetal circulation. These data lay a foundation to analyze the function of circulating MSCs during development. |
