| Primary Identifier | MGI:2664377 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Prkcq |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The floxed region encompassing about 10 kb of sequence, including a neo cassette and exons 3 and 4, was excised via cre mediated recombination in the germline. The deletion eliminated sequence encoding amino acids 10 through 87 and resulted in a frameshift mutation after codon 9. Western blot analysis indicated an absence of protein in thymocytes and CD3+ T cells. Sequence and splice sites corresponding to the testis-expressed isoform were left intact. |
