| First Author | Calderon L | Year | 2022 |
| Journal | Elife | Volume | 11 |
| PubMed ID | 35471149 | Mgi Jnum | J:324533 |
| Mgi Id | MGI:7278286 | Doi | 10.7554/eLife.76539 |
| Citation | Calderon L, et al. (2022) Cohesin-dependence of neuronal gene expression relates to chromatin loop length. Elife 11:e76539 |
| abstractText | Cohesin and CTCF are major drivers of 3D genome organization, but their role in neurons is still emerging. Here, we show a prominent role for cohesin in the expression of genes that facilitate neuronal maturation and homeostasis. Unexpectedly, we observed two major classes of activity-regulated genes with distinct reliance on cohesin in mouse primary cortical neurons. Immediate early genes (IEGs) remained fully inducible by KCl and BDNF, and short-range enhancer-promoter contacts at the IEGs Fos formed robustly in the absence of cohesin. In contrast, cohesin was required for full expression of a subset of secondary response genes characterized by long-range chromatin contacts. Cohesin-dependence of constitutive neuronal genes with key functions in synaptic transmission and neurotransmitter signaling also scaled with chromatin loop length. Our data demonstrate that key genes required for the maturation and activation of primary cortical neurons depend on cohesin for their full expression, and that the degree to which these genes rely on cohesin scales with the genomic distance traversed by their chromatin contacts. |
