| First Author | Vukovic J | Year | 2010 |
| Journal | J Leukoc Biol | Volume | 88 |
| Issue | 4 | Pages | 645-54 |
| PubMed ID | 20610801 | Mgi Jnum | J:165615 |
| Mgi Id | MGI:4837816 | Doi | 10.1189/jlb.0410194 |
| Citation | Vukovic J, et al. (2010) Bone marrow chimeric mice reveal a role for CXCR1 in maintenance of the monocyte-derived cell population in the olfactory neuroepithelium. J Leukoc Biol 88(4):645-54 |
| abstractText | Macrophages in the olfactory neuroepithelium are thought to play major roles in tissue homeostasis and repair. However, little information is available at present about possible heterogeneity of these monocyte-derived cells, their turnover rates, and the role of chemokine receptors in this process. To start addressing these issues, this study used Cxcr1(gfp) mice, in which the gene sequence for eGFP was knocked into the CXCR1 gene locus in the mutant allele. Using neuroepithelial whole-mounts from Cxcr1(gfp/+) mice, we show that eGFP(+) cells of monocytic origin are distributed in a loose network throughout this tissue and can be subdivided further into two immunophenotypically distinct subsets based on MHC-II glycoprotein expression. BM chimeric mice were created using Cxcr1(gfp/+) donors to investigate turnover of macrophages (and other monocyte-derived cells) in the olfactory neuroepithelium. Our data indicate that the monocyte-derived cell population in the olfactory neuroepithelium is actively replenished by circulating monocytes and under the experimental conditions, completely turned over within 6 months. Transplantation of Cxcr1(gfp/gfp) (i.e., CXCR1-deficient) BM partially impaired the replenishment process and resulted in an overall decline of the total monocyte-derived cell number in the olfactory epithelium. Interestingly, replenishment of the CD68(low)MHC-II(+) subset appeared minimally affected by CXCR1 deficiency. Taken together, the established baseline data about heterogeneity of monocyte-derived cells, their replenishment rates, and the role of CXCR1 provide a solid basis to further examine the importance of different monocyte subsets for neuroregeneration at this unique frontier with the external environment. |
