| First Author | Okada M | Year | 2012 |
| Journal | Biochem Biophys Res Commun | Volume | 420 |
| Issue | 2 | Pages | 479-84 |
| PubMed ID | 22450320 | Mgi Jnum | J:183546 |
| Mgi Id | MGI:5318906 | Doi | 10.1016/j.bbrc.2012.03.057 |
| Citation | Okada M, et al. (2012) DGKzeta is involved in LPS-activated phagocytosis through IQGAP1/Rac1 pathway. Biochem Biophys Res Commun 420(2):479-84 |
| abstractText | Diacylglycerol kinase (DGK) plays an important role in phosphoinositide signaling cascade by regulating the intracellular level of diacylglycerol and phosphatidic acid. The DGK family is involved in various pathophysiological responses that are mediated through unique binding partners in different tissues and cells. In this study, we identified a small GTPase effector protein, IQGAP1, as a novel DGKzeta-associated complex protein. A bacterial endotoxin, lipopolysaccharide (LPS), facilitated the complex formation in macrophages. Both proteins co-localized at the edge and phagocytic cup of the cell. Furthermore, RNA interference-mediated knockdown of DGKzeta or IQGAP1 impaired LPS-induced Rac1 activation. Primary macrophages derived from DGKzeta(-/-) mice attenuated LPS-induced phagocytosis of bacteria. These results suggest that DGKzeta is involved in IQGAP1/Rac1-mediated phagocytosis upon LPS stimulation in macrophages. |
