| First Author | Khandekar M | Year | 2007 |
| Journal | Development | Volume | 134 |
| Issue | 9 | Pages | 1703-12 |
| PubMed ID | 17395646 | Mgi Jnum | J:121242 |
| Mgi Id | MGI:3709677 | Doi | 10.1242/dev.001297 |
| Citation | Khandekar M, et al. (2007) A Gata2 intronic enhancer confers its pan-endothelia-specific regulation. Development 134(9):1703-12 |
| abstractText | GATA-2, a transcription factor that has been shown to play important roles in multiple organ systems during embryogenesis, has been ascribed the property of regulating the expression of numerous endothelium-specific genes. However, the transcriptional regulatory hierarchy governing Gata2 activation in endothelial cells has not been fully explored. Here, we document GATA-2 endothelial expression during embryogenesis by following GFP expression in Gata2-GFP knock-in embryos. Using founder transgenic analyses, we identified a Gata2 endothelium enhancer in the fourth intron and found that Gata2 regulation by this enhancer is restricted to the endocardial, lymphatic and vascular endothelium. Whereas disruption of three ETS-binding motifs within the enhancer diminished its activity, the ablation of its single E box extinguished endothelial enhancer-directed expression in transgenic mice. Development of the endothelium is known to require SCL (TAL1), and an SCL-E12 (SCL-Tcfe2a) heterodimer can bind the crucial E box in the enhancer in vitro. Thus, GATA-2 is expressed early in lymphatic, cardiac and blood vascular endothelial cells, and the pan-endothelium-specific expression of Gata2 is controlled by a discrete intronic enhancer. |
