| Primary Identifier | MGI:2675585 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cdk2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A neomycin resistance gene flanked by loxP sites was inserted into intron 3 while a third loxP site was inserted into intron 1, generating Cdk2tm2Sgo. The present allele resulted from Cre recombinase-mediated excision of exons 2 and 3 (and the selection cassette), leading to a predicted reading frame shift. Gene modification was confirmed by RT-PCR and sequencing of the resulting PCR product. |
