| First Author | Miyazaki T | Year | 1999 |
| Journal | Int Immunol | Volume | 11 |
| Issue | 8 | Pages | 1195-202 |
| PubMed ID | 10421777 | Mgi Jnum | J:56762 |
| Mgi Id | MGI:1342392 | Doi | 10.1093/intimm/11.8.1195 |
| Citation | Miyazaki T, et al. (1999) Lambda5 is required for rearrangement of the Ig kappa light chain gene in pro-B cell lines. Int Immunol 11(8):1195-202 |
| abstractText | Lambda5 associates with V(pre-B) to form the surrogate light (L) chain. The phenotype of lambda5 knockout mice showed severe impairment of B cell development from pro-B to immature B cell stages. To investigate the function of the surrogate L chain at this stage, we restored expression of lambda5 to lambda5-deficient pro-B cell lines which were established from bone marrow cells of lambda5 knockout mice in the presence of IL-7 and a stromal cell line. Some of these lines are severely impaired in B cell development from pro-B to immature B cell stages as is seen in vivo in lambda5 knockout mice. Restoration of lambda5 protein by retroviral-mediated gene transfer into established lambda5-deficient pro-B cell lines induced rearrangement of the Ig kappa L chain genes after removal of IL-7 from the culture. Immunoprecipitation revealed that the restored lambda5 in the cell line is coupled with V(pre-B) to form the surrogate L chain. The results demonstrate that formation of a complete surrogate L chain, consisting of both lambda5 and V(pre-B), stimulates efficient rearrangement of the kappa L chain genes. |
