| Primary Identifier | MGI:2677969 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Sftpd |
| Transmission | Germline | Strain of Origin | C57BL/6J x (Rb(11.16)2H x Rb(16.17)32Lub)F1 |
| Is Recombinase | false | Is Wild Type | false |
| description | This allele was generated in ES cells containing Sftpa1tm2Haw. |
| molecularNote | Using ES cells in which a correctly targeted modification of the Sftpa gene had already been created, exon 2 along with the translation start site, was replaced with a PGK-neomycin cassette. Northern blot analysis demonstrated the absence of any Sftpa mRNA in any doubly homozygous mice as well as the absence of full length Sftpd mRNA. A small amount of truncated mRNA for Sftpd lacking the translation start site was seen. Western blot analysis confirmed that no protein products were produced. |
