| Primary Identifier | MGI:2680287 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mapkapk5 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 6 was replaced with a neo cassette. The targeted exon encoded amino acids 131 through 161 which make up portions of the kinase catalytic core subdomains VIa and VIb. Northern blot and RT-PCR analyses identified mutant transcript approximately 100 bp shorter than normal transcript. Sequence analysis showed the mutant transcript to contain neither exon 6 nor the neo cassette. Low levels of truncated protein were detected by Western blot analysis, but this protein was reported to lack catalytic activity. |
