| First Author | Chattopadhyay S | Year | 2013 |
| Journal | MBio | Volume | 4 |
| Issue | 2 | PubMed ID | 23532979 |
| Mgi Jnum | J:275978 | Mgi Id | MGI:6304075 |
| Doi | 10.1128/mBio.00636-12 | Citation | Chattopadhyay S, et al. (2013) Inhibition of viral pathogenesis and promotion of the septic shock response to bacterial infection by IRF-3 are regulated by the acetylation and phosphorylation of its coactivators. mBio 4(2) |
| abstractText | Interferon (IFN) is required for protecting mice from viral pathogenesis; reciprocally, it mediates the deleterious septic shock response to bacterial infection. The critical transcription factor for IFN induction, in both cases, is IRF-3, which is activated by TLR3 or RIG-I signaling in response to virus infection and TLR4 signaling in response to bacterial infection. Here, we report that IRF-3's transcriptional activity required its coactivators, beta-catenin and CBP, to be modified by HDAC6-mediated deacetylation and protein kinase C isozyme beta (PKC-beta)-mediated phosphorylation, respectively, so that activated nuclear IRF-3 could form a stable transcription initiation complex at the target gene promoters. beta-Catenin bridges IRF-3 and CBP, and the modifications were required specifically for the interaction between beta-catenin and CBP but not beta-catenin and IRF-3. Consequently, like IRF-3(-/-) mice, HDAC6(-/-) mice were resistant to bacterial lipopolysaccharide-induced septic shock. Conversely, they were highly susceptible to pathogenesis caused by Sendai virus infection. Thus, HDAC6 is an essential component of the innate immune response to microbial infection. |
