| First Author | Clybouw C | Year | 2011 |
| Journal | Blood | Volume | 118 |
| Issue | 15 | Pages | 4120-8 |
| PubMed ID | 21868573 | Mgi Jnum | J:178386 |
| Mgi Id | MGI:5298285 | Doi | 10.1182/blood-2011-04-347096 |
| Citation | Clybouw C, et al. (2011) Regulation of memory B-cell survival by the BH3-only protein Puma. Blood 118(15):4120-8 |
| abstractText | Apoptosis is crucial for immune system homeostasis, including selection and survival of long-lived antibody-forming cells and memory cells. The interactions between proapoptotic and pro-survival proteins of the Bcl-2 family are critical for this process. In this report, we show that expression of the proapoptotic BH3-only Bcl-2 family member Puma was selectively up-regulated on in vitro activation with antigens or mitogens of both human and mouse B cells. Puma expression coincided in vivo, with the prosurvival Bcl-2 family member Mcl-1 within the germinal centers and its expression correlates with the germinal center like phenotype of Burkitt lymphoma. Experiments performed in Puma-deficient mice revealed that Puma is essential for apoptosis of mitogen-activated B cells in vitro and for the control of memory B-cell survival. In conclusion, using both human and murine models, our data show that Puma has a major role in the T cell- dependent B-cell immune response. These data demonstrate that Puma is a major regulator of memory B lymphocyte survival and therefore a key molecule in the control of the immune response. |
