Primary Identifier | MGI:2681693 | Allele Type | Targeted |
Gene | Rag1 | Transmission | Germline |
Strain of Origin | 129S6/SvEvTac | Is Recombinase | false |
Is Wild Type | false |
molecularNote | The Rag1 coding sequence was replaced with a truncated core Rag-1 sequence followed by a floxed neomycin resistance gene. The neomycin gene was then removed from properly targeted ES cells by transient transfection with a plasmid based Cre recombinase. Core Rag1 was expressed at levels comparable to that found for intact protein. |