| Primary Identifier | MGI:2682554 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Slc6a5 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A 2.1 kb genomic fragment encompassing the exons encoding the fourth transmembrane domain was replaced with a neomycin selection cassette inserted by homologous recombination. Neither transcript nor protein was detected by RT-PCR or Western blot analyses of homozygous mutant brain stem tissue. The absence of protein was also verified in fractions obtained from mutant spinal cord extracts. |
