| First Author | Li X | Year | 2021 |
| Journal | Cell Death Dis | Volume | 12 |
| Issue | 6 | Pages | 553 |
| PubMed ID | 34050134 | Mgi Jnum | J:315771 |
| Mgi Id | MGI:6813300 | Doi | 10.1038/s41419-021-03846-x |
| Citation | Li X, et al. (2021) Neocortex- and hippocampus-specific deletion of Gabrg2 causes temperature-dependent seizures in mice. Cell Death Dis 12(6):553 |
| abstractText | Mutations in the GABRG2 gene encoding the gamma-aminobutyric acid (GABA) A receptor gamma 2 subunit are associated with genetic epilepsy with febrile seizures plus, febrile seizures plus, febrile seizures, and other symptoms of epilepsy. However, the mechanisms underlying Gabrg2-mediated febrile seizures are poorly understood. Here, we used the Cre/loxP system to generate conditional knockout (CKO) mice with deficient Gabrg2 in the hippocampus and neocortex. Heterozygous CKO mice (Gabrg2(fl/wt)Cre(+)) exhibited temperature-dependent myoclonic jerks, generalised tonic-clonic seizures, increased anxiety-like symptoms, and a predisposition to induce seizures. Cortical electroencephalography showed the hyperexcitability in response to temperature elevation in Gabrg2(fl/wt)Cre(+) mice, but not in wild-type mice. Gabrg2(fl/wt)Cre(+) mice exhibited spontaneous seizures and susceptibility to temperature-induced seizures. Loss of neurons were observed in cortical layers V-VI and hippocampus of Gabrg2(fl/wt)Cre(+) mice. Furthermore, the latency of temperature- or pentylenetetrazol-induced seizures were significantly decreased in Gabrg2(fl/wt)Cre(+) mice compared with wild-type mice. In summary, Gabrg2(fl/wt)Cre(+) mice with Gabrg2 deletion in the neocortex and hippocampus reproduce many features of febrile seizures and therefore provide a novel model to further understand this syndrome at the cellular and molecular level. |
