| First Author | Chang D | Year | 2023 |
| Journal | Front Immunol | Volume | 14 |
| Pages | 1105145 | PubMed ID | 36969227 |
| Mgi Jnum | J:335145 | Mgi Id | MGI:7449795 |
| Doi | 10.3389/fimmu.2023.1105145 | Citation | Chang D, et al. (2023) A cis-element at the Rorc locus regulates the development of type 3 innate lymphoid cells. Front Immunol 14:1105145 |
| abstractText | BACKGROUND: As an important early source of IL-17A and IL-22 in immune responses, type 3 innate lymphoid cells (ILC3s) are critically regulated by the transcription factor retinoic-acid-receptor-related orphan receptor gamma t (RORgammat). Previously, we have identified a crucial role of the conserved non-coding sequence 9 (CNS9), located at +5,802 to +7,963 bp of the Rorc gene, in directing T helper 17 differentiation and related autoimmune disease. However, whether cis-acting elements regulate RORgammat expression in ILC3s is unknown. RESULTS: Here we show that CNS9 deficiency in mice not only decreases ILC3 signature gene expression and increases ILC1-gene expression features in total ILC3s, but also leads to generation of a distinct CD4(+)NKp46(+) ILC3 population, though the overall numbers and frequencies of RORgammat(+) ILC3s are not affected. Mechanistically, CNS9 deficiency selectively decreases RORgammat expression in ILC3s, which thus alters ILC3 gene expression features and promotes cell-intrinsic generation of CD4(+)NKp46(+) ILC3 subset. CONCLUSION: Our study thus identifies CNS9 as an essential cis-regulatory element controlling the lineage stability and plasticity of ILC3s through modulating expression levels of RORgammat protein. |
