| Primary Identifier | MGI:2686794 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Rho)1Wbae |
| Strain of Origin | C57BL/6 x SJL | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The transgenic construct contained a 15 kb genomic fragment encompassing the mouse rhodopsin gene. 3 substitution mutations were engineered in exon 1 (V20G, P23H, and P27L). 6 kb of upstream sequence and 3 kb of downstream sequence were included in the construct. Northern blot analysis showed that both the inserted mutant rhodopsin transcript and the wild-type transcript are present in transgenic mice, indicating that the site of integration is not at the endogenous rhodopsin locus. |
