| First Author | Kyriakides C | Year | 1999 |
| Journal | J Appl Physiol (1985) | Volume | 87 |
| Issue | 6 | Pages | 2357-61 |
| PubMed ID | 10601189 | Mgi Jnum | J:103341 |
| Mgi Id | MGI:3609263 | Doi | 10.1152/jappl.1999.87.6.2357 |
| Citation | Kyriakides C, et al. (1999) Membrane attack complex of complement and neutrophils mediate the injury of acid aspiration. J Appl Physiol 87(6):2357-61 |
| abstractText | A significant role for the alternative complement pathway in acid aspiration has been demonstrated by the observation that C3 genetic knockout mice are protected from injury. Utilizing C5-deficient mice, we now test the role of the terminal complement components in mediating injury. Lung permeability in C5-deficient mice was 64% less than in wild-type animals and was similar to wild-type mice treated with soluble complement receptor type 1, which gave a 67% protection. Injury was fully restored in C5-deficient mice reconstituted with wild-type serum. The role of neutrophils was established in immunodepleted wild-type animals that showed a 58% protection. Injury was further reduced (90%) with the addition of soluble complement receptor type 1, indicating an additive effect of neutrophils and complement. Similarly, an additional protection was noted in C5-deficient neutropenic mice, indicating that neutrophil-mediated injury does not require C5a. Thus acid aspiration injury is mediated by the membrane attack complex and neutrophils. Neutrophil activity is independent of C5a. |
