| First Author | Romanienko PJ | Year | 2000 |
| Journal | Mol Cell | Volume | 6 |
| Issue | 5 | Pages | 975-87 |
| PubMed ID | 11106738 | Mgi Jnum | J:65881 |
| Mgi Id | MGI:1927413 | Doi | 10.1016/s1097-2765(00)00097-6 |
| Citation | Romanienko PJ, et al. (2000) The mouse spo11 gene is required for meiotic chromosome synapsis. Mol Cell 6(5):975-87 |
| abstractText | The Spo11 protein initiates meiotic recombination by generating DNA double-strand breaks (DSBs) and is required for meiotic synapsis in S. cerevisiae. Surprisingly, Spo11 homologs are dispensable for synapsis in C. elegans and Drosophila yet required for meiotic recombination. Disruption of mouse Spo11 results in infertility. Spermatocytes arrest prior to pachytene with little or no synapsis and undergo apoptosis. We did not detect Rad51/Dmc1 foci in meiotic chromosome spreads, indicating DSBs are not formed. Cisplatin-induced DSBs restored Rad51/Dmc1 foci and promoted synapsis. Spo11 localizes to discrete foci during leptotene and to homologously synapsed chromosomes. Other mouse mutants that arrest during meiotic prophase (Atm -/-, Dmc1 -/-, mei1, and Morc(-/-)) showed altered Spo11 protein localization and expression. We speculate that there is an additional role for Spo11, after it generates DSBs, in synapsis. |
