| Primary Identifier | MGI:3028868 | Allele Type | Targeted |
| Attribute String | Hypomorph | Gene | Pou2f1 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 3 was replaced with a floxed neo cassette. The deleted exon is common to all three known isoforms and does not overlap with Cd247, a locus that overlaps a distal portion of the Pou2f1 locus. Mutant transcript, in which exon 2 aberrantly splices to exon 4a, was identified by RT-PCR analysis of homozygous mutant MEFs. The aberrant splicing results in a frameshift mutation which consequently generates a nonsense mutation and precludes the translation of two-thirds of the mature protein including the DNA-binding domain. Supershift analysis of DNA binding assays using nuclear extracts from homozygous cells detected very low levels of protien, thus this is a severely hypomorphic allele. |
