| First Author | Ninkina NN | Year | 1995 |
| Journal | Brain Res Mol Brain Res | Volume | 33 |
| Issue | 2 | Pages | 233-44 |
| PubMed ID | 8750882 | Mgi Jnum | J:29396 |
| Mgi Id | MGI:76920 | Doi | 10.1016/0169-328x(95)00128-f |
| Citation | Ninkina NN, et al. (1995) Nerve growth factor-regulated properties of sensory neurones in Oct-2 null mutant mice. Brain Res Mol Brain Res 33(2):233-44 |
| abstractText | The POU-domain transcription factor Oct-2 is expressed in both B lymphocytes and sensory neurones, where its expression is regulated by nerve growth factor (NGF), In order to define a possible role for Oct-2 in neurotrophin signalling, we examined the expression of an NGF-regulated channel (capsaicin-evoked ion fluxes), neuropeptides (substance P, calcitonin gene-related peptide), structural proteins (neurofilaments and peripherin) and receptors (trks) in dorsal root ganglion neurones derived from perinatal transgenic mice containing a defective Oct-2 structural gene. Northern blots show that central nervous tissue contains a larger than normal(> 10 kb) mRNA transcript corresponding in size to an Oct-2 transcript encoding a defective protein. PCR analysis shows the absence of normal Oct-2 transcripts in dorsal root ganglia. In null mutants, capsaicin sensitivity, and neuropeptide and cytoskeletal protein expression were unaffected by the loss of Oct-2 expression. The number of sensory neurones and the gross morphology of CNS tissues that normally express high levels of Oct-2 were also examined and found to be normal in the null mutant. Heterozygous animals show normal thresholds of sensitivity to noxious heat and normal inflammatory responses. Oct-2 does not therefore play an essential role in the NGF responsiveness of sensory neurones in these animals. |
