| Primary Identifier | MGI:3029984 | Allele Type | Targeted |
| Attribute String | Hypomorph, Inserted expressed sequence | Gene | Runx1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A cDNA encoding a C-terminally truncated RUNX1 protein was inserted into exon 4 of the endogenous locus via homologous recombination. The encoded protein truncates at residue 446 and thereby retains the trans-activating subdomains and lacks the VWRPY motif. Semi-quantitative RT-PCR analysis of splenic and thymic message showed the levels of mutant transcript in homozygous mutant mice to be similar to the levels of normal transcript in wild-type mice. |
